The business group says there should be an incentive to refurbish neglected Georgian buildings on Denny Street and Day Place.
It says the refurbishment would allow small businesses to open offices there.
Tralee Chamber Alliance is based on Denny Street.
CEO Kieran Ruttledge says it has plans to refurbish a floor on its premises to help start-ups.
This would be done on a not-for-profit basis.
Mr Ruttledge says Kerry’s new TDs need to do all they can to support the county’s biggest town.