The single vehicle collision occurred at 3.20 yesterday afternoon on the N86 between Ballintaggart and Dingle.
The man was injured when the minibus he was driving left the road and struck a ditch.
Four passengers travelling in the minibus were not injured.
The man was taken by ambulance to University Hospital Kerry, where he was later pronounced dead.
The local coroner was notified and Garda forensic collision investigators attended the scene.
The road had been closed for a time but has since reopened.
Gardaí are appealing for witnesses to contact Tralee Garda Station 066 710 2300 or the Garda Confidential Line 1800 666 111.